Laserchrom HPLC - HPLC Solvent Guide

Introduction for non-chemists . Organic solvents fall into classes, such as alkanes, alcohols, acids, aldehydes, etc where each member of the class ha...

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HPLC troubleshooting for changing baseline, tailing peaks, and pump and detector problems.

3 Troubleshooting Case Study A Few Clicks to Find the Probable Cause Using the problem of negative peaks described above, the HPLC Troubleshooting …

Nov 09, 2013 · Reverse phase chromatography is the most commonly used LC or HPLC separation mode.It is used to separate nonpolar molecules in solution.In reverse phase the stationary phase is nonpolar and the mobile phase is polar.The name “reversed

Nov 01, 2013 · normal phase liquid chromatography, polar, stationary phase, nonpolar, table i.1, properties, mobile s phase, retained solute, order of elution, what is …

HPLC Troubleshooting Guide. 2 Quick Reference Tables If you have a problem you need to solve now, use these Troubleshooting Tables. ... Check and adjust pump flow rate (see 2.4) Column overloaded 1. Reduce amount of sample injected 2

compensation software which is found in most modern HPLC systems. Many of these systems will allow you to manually enter the actual liquid compressibility values for each liquid (pump channel) used. This can result in better baseline stability and le

3 Figure B. Supelguard Columns Prolong the Lifespan of Your Analytical Columns column: SUPELCOSIL LC-PCN, 25 cm x 4.6 mm I.D., 5 μm particles (with

A Troubleshooting Guide – Version 1.1. 2 1. Introduction 4 1.1 Purpose 4 1.2 Content 4 2. ... system pressure under normal operating conditions each day or each time your HPLC is used. ... pump manual. 5. Loosen detector waste outlet fitting.

HPLC Column Selection Guide Column Parameters Internal Diameter An important parameter of a HPLC column is the internal diameter (ID) as this directly influences the